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Benchwork accuracy and throughput by Hugh Dimmersteg - Thu, 06 Nov 2014 22:36:18 EST ID:zrV5jqLv
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Are there any standards w/r/t benchwork accuracy and throughput? E.g. If I take 3 days to extract RNA from 69 muscle samples and 2 of them have to be redone (97%), is this good/bad?

jolly african-american by Barnaby Wozzlemere - Mon, 03 Nov 2014 21:12:12 EST ID:/RojIEH7
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gonna mount an outside yagi antenna for over-the-air tv. I know how to mount it to the house but I was wondering about lightning safety. Will grounding to a 8 ft long copper rod in the ground be adequate? My main concern is not having lightning travel through the coax into the house destroying tvs, connected electronics, or worse.
 >> Phineas Chinkinchack - Tue, 04 Nov 2014 00:30:44 EST ID:RK6dBCZl Ignore Report Quick Reply >>75652It needs to be grounded, and there are some.. fuse/breaker things you can get between the antenna and equipment. I forget what they're called.
 >> Barnaby Wozzlemere - Tue, 04 Nov 2014 18:55:06 EST ID:/RojIEH7 Ignore Report Quick Reply >>75653is that really effective? lightning can have voltages and amps higher than 1 million. what's stopping lightning from just jumping the fuse/breaker gap?
 >> Angus Sicklefedge - Wed, 05 Nov 2014 10:29:12 EST ID:RK6dBCZl Ignore Report Quick Reply >>75654They're better when placed close to the antenna, not close to the appliance, not close to the appliance. They're much bigger than a typical fuse/breaker gap. AND the antenna still needs to be properly grounded for it to work.I don't have much experience with antennae, so don't take my word for it. However, I believe grounding is sufficient to protect against most of the fire hazard. The surge protector is then needed to protect appliances, any fire hazard that remains.This has lots of info: http://www.arrl.org/lightning-protection The weblinks section is probably what you want.
 >> Angus Sicklefedge - Wed, 05 Nov 2014 10:33:37 EST ID:RK6dBCZl Ignore Report Quick Reply >>75655Also, if those links are too dense, search the ham forums for a general guide.
 >> Doris Hangerfield - Wed, 05 Nov 2014 19:36:13 EST ID:/RojIEH7 Ignore Report Quick Reply http://www.k3dav.com/antlightningprotectors.htm

Meme vector theory by Eliza Trotham - Mon, 03 Nov 2014 01:00:32 EST ID:JUs0i9Nd
1414994432415.jpg -(26177 B, 883x136) Thumbnail displayed, click image for full size.
Some of you more savvy /chem/ers may remember this from a year ago. The meme equation. Well, I thought about it some more and pic related is what I came up with. M=some constant related to the population's resistance to the meme, B=some constant related to the meme's intrinsic value/spreadability

Here's js for the equation in the pic: "$\frac{1}{\left(\frac{\left(\frac{1}{1+e^{-300000000\left(\frac{M-B}{\sqrt{\left(M-B\right)x^2+300000000}}\right)}}\right)\left(0.0001\right)+\left(\frac{1}{1+e^{-300000000\left(\frac{M-B}{\sqrt{\left(M-B\right)x^2+300000000}}\right)}}\right)\left(M-B\right)^2}{Bx^B}\right)^{\frac{\left(M\right)}{B}}+1}$"

You can play around with it here: https://www.desmos.com/calculator

It models the number of meme vectors for a give meme over time. What's a meme vector? A dynamic, singular entity capable of passing a meme onto another entity.

Still no empirical data to model to it but I hope that changes soon. I have a couple pages of theory typed up. Any questions or suggestions would be hella appreciated.
 >> Alice Chimblekick - Mon, 03 Nov 2014 10:42:46 EST ID:uGD5aNS6 Ignore Report Quick Reply >>75643lol why man?
 >> Eliza Trotham - Mon, 03 Nov 2014 10:47:43 EST ID:JUs0i9Nd Ignore Report Quick Reply >>75644If you know the meme strength and the population resistance you can predict the pattern of meme spreading
 >> James Fackleman - Mon, 03 Nov 2014 20:28:47 EST ID:tCxc3jCe Ignore Report Quick Reply You forgot to factor in the aliens

Creating a human by Ernest Callyforth - Sun, 26 Oct 2014 23:31:12 EST ID:kEuMwRGV
1414380672820.jpg -(64352 B, 640x360) Thumbnail displayed, click image for full size.
Toss the ingredients together in the large hadron collider...
3 posts omitted. Click Reply to view.
 >> Matilda Cambleham - Mon, 27 Oct 2014 19:22:03 EST ID:YxJntRdd Ignore Report Quick Reply 1414452123725.jpg -(7158 B, 297x170) Thumbnail displayed, click image for full size. FAGHER Y U MAEK ME??KILL MEEE
 >> Basil Drinnermick - Tue, 28 Oct 2014 22:32:51 EST ID:uGD5aNS6 Ignore Report Quick Reply >>75620toss a bunch of legos into a pot and get legoland, right?
 >> Alice Pavingfack - Tue, 28 Oct 2014 22:36:47 EST ID:RK6dBCZl Ignore Report Quick Reply >>75627This sounds like one of those ridiculous creationist arguments.
 >> Phoebe Pittson - Wed, 29 Oct 2014 18:24:22 EST ID:cM6rhCgJ Ignore Report Quick Reply >>75628No, just like you can't get a human without building it, you can't get lego land without building it. You can say you don't believe in GOD now but just wait til you're on your death bed."There's no atheists in foxholes"The PopeYou'll see the truth soon, and GOD and Jesus still love you.
 >> Fuck Clundlelure - Wed, 29 Oct 2014 21:16:15 EST ID:uGD5aNS6 Ignore Report Quick Reply 1414631775844.jpg -(32967 B, 480x360) Thumbnail displayed, click image for full size. >>75633>>75628Da fuq???Not at all what I meant. Not creationist, just meant you can't toss a bunch of elements into something and expect to get a human out of it. But that doesn't fucking mean a God did it, fuck off with your religious bullshit. Your god is a product of your mind, a hallucination.Regardless, I meant it just takes a lot of time and a lot of luck for life to have been generated from raw elements, and the right conditions (like UV).

Trying to find genes by Shitting Drebbermire - Wed, 29 Oct 2014 04:19:59 EST ID:1LIN4zeR
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I'm doing this assignment at the moment where, part of it involves finding the normal function of a given set of genes. I've only been given a Gene ID but I just can't seem to find how I can use this ID on the net in order to find out my genes. I've tried a whole bunch of gene databases but nothing comes up.

To give some examples, some gene ID's are LS0109, U272, and U355. Does anyone know where I can go and pop these ID's in and get back some answers? Or just what I'm supposed to do with them in general?

I feel stupid because this is something I think shouldn't be hard but I'm having a hard time at it.
 >> Rebecca Brenderlerk - Wed, 29 Oct 2014 07:27:39 EST ID:KH/wMTAN Ignore Report Quick Reply http://www.ncbi.nlm.nih.gov/nucest/105635198These are cDNA. Just select "All database" and type the name of the gene.
 >> Matilda Mellycocke - Sat, 01 Nov 2014 08:42:32 EST ID:lb8TtRsW Ignore Report Quick Reply >>75631Thank you very much.nb.

Temperature logging by Charles Bambleville - Wed, 15 Oct 2014 15:09:13 EST ID:vJr4oEuE
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So i want to log some temperature.
Temperature range measured: ~ -50 - 50°C
Duration: maximum of 5 hrs

So what i seek is a matching thermocouple. I have a NiCr-Ni (K) here but as far i know the tolerance of these are awful in the desired range. Is this right?

What other options would be availible? I was thinking about some Cu-CuNi (T) thermocouples, would they get me a better performance? Is there any difference between some for 20$and some for 50$?
1 posts omitted. Click Reply to view.
 >> Eugene Pepperbanks - Thu, 16 Oct 2014 12:34:14 EST ID:IkHTG/Ep Ignore Report Quick Reply >>75545I've just found a Voltcraft Datalogger with 4 inputs for K-type thermocouples lying around in one of our labs. For the moment i'll just use these as I also have some thermocoulpes for it and it won't allow me to use T-type one's.I don't need the exact range, my measured range should just be somwhat in the possible range with a tolerable accuracy.I don't really want to put something together myself as I am not very skilled in that area and i need it fast. I actually want to measure the core temp of dough while shock-frosting it thus I need something i can stick in there.I'll make my first tests and will report back.
 >> Isabella Chorrybet - Thu, 16 Oct 2014 17:54:20 EST ID:f5OSHUfT Ignore Report Quick Reply >>75548In that case, just shop around for one in the right range. Look at the datasheet for the thermocouple, because it might tell you how much the accuracy suffers outside of its normal operating range. For example, maybe under -30C it will vary by +/- 2C instead of +/- 1. Then, if you're graphic it you can add the appropriate error bars.
 >> Isabella Chorrybet - Thu, 16 Oct 2014 17:57:18 EST ID:f5OSHUfT Ignore Report Quick Reply >>75550Just to emphasize: look at the datasheet. Find and read the datasheet for the thermocouple you already have. if it has a company and model number, you can definitely find it.
 >> Simon Lightson - Wed, 22 Oct 2014 11:44:22 EST ID:qEOCjdzP Ignore Report Quick Reply 1413992662173.png -(20445 B, 408x265) Thumbnail displayed, click image for full size. >>75551I dont have a datasheet. My prof bought these thermocouples a few years ago in bulk (like 10 for 15€) and there is nothing written on them. He suggested to calibrate them manually with a substance that freezes at our desired temp. . I'll try that out.pic related, grah i made last time of the air in the freezer (just ignore legend entrys)
 >> Phineas Poblingmig - Sat, 25 Oct 2014 18:08:54 EST ID:2dBy3g3n Ignore Report Quick Reply Could you use a more traditional resistor? Thermocouples are designed more to be directly integrated into a circuit, while if you used a high ohm resistor, mosfet, and a quick calibration, it seems you could get much better results. If the resistor was small enough, an op-amp could probably be used. I have a \$20 usb board which can handle 8-bit 0-5v signals, and it's a generic dev thing with C (closed and open source), Python (open source), and VisualBasic or somethin (.Net)Even better, you could make a voltage or current controlled 555 timer, and do a pulse-per-second method for greater resolution.

Nobel by Samuel Sottingfudging - Fri, 24 Oct 2014 02:52:07 EST ID:t/vh2uWX
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Which field of physics has the most opportunities for winning the Nobel Prize?
I personally would guess that it's cosmology, but I only know Newtonian, Lagrangian and Hamiltonian classical mechanics.
 >> trypto - Fri, 24 Oct 2014 09:50:33 EST ID:RK6dBCZl Ignore Report Quick Reply >>75614Probably biophysics, so you could get a nobel in medicine/physiology, or even chemistry if you determine the structure of some important protein.Materials science is another fruitful area.

msm crystallization question. by erradick addik - Tue, 21 Oct 2014 19:38:30 EST ID:1Cm3qiFx
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anyone have any general tips or techniques on making perfect msm crystals from joint pills?
the ones i made last time i spent a lot of time experimenting withought paying good attention. that being said, ive got em disolved in water, the water is a gnarly brown color and i want clear crystals like last time!
 >> Ernest Pebblestetch - Tue, 21 Oct 2014 22:00:29 EST ID:f5OSHUfT Ignore Report Quick Reply >>75596I've never done it. And I don't even know what msm is. However, I know basic crystalization techniques.Also, do it slowly. Let it slowly cool to room temp. Then cool it in cold water for a while. Then freeze it. The slower the better. If you do it quickly, then the impurities get trapped within the crystal. If you do it slowly, the crystals exclude the impurities.Rinse and repeat. Literally. Rinse the crystals a bit with the cold solvent, and repeat the crystalization with fresh solvent. The idea is that as you crystalize, the impurities are left in the solution, and the pure crystals precipitate out. Sometimes it takes a few tries. But, if you see no improvement, then it's possible the impurity won't be removed by crystalization.You could also try filtering over carbon black to remove the colored shit.Be careful, though. Main Stream Media can make you retarded.
 >> Ernest Pebblestetch - Tue, 21 Oct 2014 22:07:49 EST ID:f5OSHUfT Ignore Report Quick Reply Waaaaaaaaaaaaaaait a second. I googled it, and you're just crystalizing DMSO? WTF? It's a liquid at room temp. You're not going to get DMSO crystals (I'd never heard it called msm before, and I'm not about to start).If you got crystals like in your pic (which didn't immediately melt in your your hand), then you crystalized something else entirely. My advice remains. Check out the ingredients to find out what you crystalized.That is, assuming I'm reading this correctly.And, just to be sure: you're not smoking this shit, are you? Because that's how /chem/ usually works. People come asking how to make crystals to smoke.
 >> Alice Brookshit - Thu, 23 Oct 2014 10:12:03 EST ID:uGD5aNS6 Ignore Report Quick Reply >>75601Its DMSO2, not dimethyl sulfoxide.MSM is a common ingredient in joint pain supplements, along with glucosamine and chondritin
 >> trypto - Thu, 23 Oct 2014 23:55:40 EST ID:RK6dBCZl Ignore Report Quick Reply >>75605Ahhh. Gotcha. I googled msm, saw DMSO and stopped reading.OP, my general crystalization advice remains. As well as the advice not to vape it.
 >> Sidney Babbleville - Fri, 24 Oct 2014 09:27:15 EST ID:uGD5aNS6 Ignore Report Quick Reply >>75608lol only on 420chan would you need to make that warning explicitly said. gotta love this place

Building a Separatory Funnel by Cedric Cimmleman - Thu, 28 Aug 2014 09:26:59 EST ID:tCxc3jCe
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How would you guys build a homemade separatory funnel? I want to make one I can fit a gallon in for winemaking, but the 4000 ml ones are like 400 bucks. How would you guys build one?
5 posts omitted. Click Reply to view.
 >> erradick addik - Tue, 21 Oct 2014 19:40:16 EST ID:1Cm3qiFx Ignore Report Quick Reply 1413934816201.jpg -(11099 B, 183x276) Thumbnail displayed, click image for full size. >>75252ziplock baggie
 >> erradick addik - Tue, 21 Oct 2014 19:46:17 EST ID:1Cm3qiFx Ignore Report Quick Reply >>75252or a space bag 0.o
 >> Thomas Sudgedock - Fri, 24 Oct 2014 00:00:11 EST ID:tCxc3jCe Ignore Report Quick Reply >>75595>>75597>>75598OP hereLOL. Im all about improvised ghetto equipment but really guys? Maybe its the mushs but this is extremely funny to me. I enjoy that idea. I would maybe possibly (probably not though) use that for wine making in a pinch, but other than that I would never put a solvent other than water inside a ziplock bag. I ended up just buying a sep funnel. But the glass carboy one I build works well too.
 >> trypto - Fri, 24 Oct 2014 00:12:29 EST ID:RK6dBCZl Ignore Report Quick Reply >>75609You never answered why you need one for wine making. It's not just getting rid of lees, is it? Or are you doing some analysis?
 >> Thomas Sudgedock - Fri, 24 Oct 2014 00:24:51 EST ID:tCxc3jCe Ignore Report Quick Reply >>75612Yeah I use it for getting rid of any precipitate and gunk that's in the solution instead of running it through a filter like cheesecloth, aka racking. I realize that its not the most efficient way. I mostly use it for other projects anyways.

whattup chem by Matilda Fazzlepin - Tue, 02 Sep 2014 00:20:22 EST ID:GBHOrOti
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so I want to learn chemistry, but I work all the time and don't have time to go to school. Do you guys know of any, like.. online courses or textbooks I can use to learn about basic chemistry?
2 posts omitted. Click Reply to view.
 >> Alice Daddlewill - Tue, 02 Sep 2014 00:30:56 EST ID:leb0yeuz Ignore Report Quick Reply >>75312Khan Academy is supposed to be good.
 >> Shit Breffingwell - Tue, 02 Sep 2014 15:36:28 EST ID:RqP3t9oX Ignore Report Quick Reply >>75312http://www.chemguide.co.uk/Tailored to the UK A-level syllabus, I used it a lot when my books couldn't help, there isn't much help with calculations on the site though, because the guy who does the site made his own textbook for all of the math stuff (which is good, try to get ahold of it if you can't into math.)
 >> Reuben Duckford - Tue, 23 Sep 2014 10:17:01 EST ID:8JJsH6mv Ignore Report Quick Reply >>75312openculture.com/freeonlinecoursesctrl + f, 'chemistry' and there are like 30 courses thereenjoy. share the link, please.
 >> Samuel Dartshaw - Sun, 28 Sep 2014 14:38:25 EST ID:ILxqROob Ignore Report Quick Reply www.khanacademy.org has the info that's about equal to an associates in science, it's free and very well explained videos
 >> Nicholas Bardbanks - Sun, 19 Oct 2014 07:45:56 EST ID:NH4UxYqY Ignore Report Quick Reply http://www.openculture.com/freeonlinecourseshttp://physicsdatabase.com/book-list-by-title/http://www.math.unl.edu/~jump/Center1/BioChemLabs.html

Theoretical physics books by Hamilton Chinkintock - Fri, 17 Oct 2014 13:22:46 EST ID:l+0PKAWH
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Any good beginner theoretical physics books which only include the formulas and definitions, and have as little descriptions as possible? I want a list of definitions which I can research myself, and find best explanations myself on the Internet.
 >> David Fobblelidge - Sun, 19 Oct 2014 00:50:45 EST ID:A2UoWeAl Ignore Report Quick Reply This sounds like what you're asking for:http://nguyenquangdong.vatly.net/files/2012/11/BANG-TOM-TAT-CONG-THUC-VAT-LY.pdf
 >> Nicholas Bardbanks - Sun, 19 Oct 2014 07:40:08 EST ID:NH4UxYqY Ignore Report Quick Reply >>75558http://quantizd.blogspot.ca/p/so-you-want-to-become-physicist.html

Whats your science job? by Basil Pizzlefuck - Fri, 15 Aug 2014 22:12:48 EST ID:IKfzuTsW
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How many of you guys actually have science degrees and jobs within the science industry? What do you do and do you enjoy it?

I've got a year left of my chemistry degree, with a job lined up at the end of it in a company that produces and designs biocatalysts. (assuming I get the grades) The plan is to do postgrad into catalyst research eventually but I want to fund myself instead of loans so I want to work for a few years before hand.

I really enjoy inorganic chem, but most inorganic catalyst are expensive and rare and toxic ect and with this big push for green chemistry, biocatalyst seems like a good industry to get into.
30 posts and 1 images omitted. Click Reply to view.
 >> Fuck Nunkingold - Sat, 18 Oct 2014 21:10:46 EST ID:f5OSHUfT Ignore Report Quick Reply >>75571OH yeah, and you can apply to real tech jobs as you're also applying to grad school. Grad school can be a fall-back option for you.Make sure you meet the requirements though. Publications don't mean shit if you only have one academic letter of rec.
 >> Clara Dondlestone - Sat, 18 Oct 2014 23:23:46 EST ID:V5qF06Hs Ignore Report Quick Reply >>75573>>75574>>75575ugh dammit. i really don't want to do grad school.well, i mean, i would actually like doing a master's. but i've been told those are just as bad as BS. getting a PhD and going into academia after isn't something i ever planned to do and it sounds like pure torture to me. i like the subject but needing to constantly know absolutely every new bit of info that comes out -- fed through the Boring Scientific Paper format -- and having to compete with other people for a nip at the government's grant money teat seems like a horrible job.i would really love the job i have now if i didn't have the prospect of needing a stupid PhD to get a job (after this one) hanging over my head. i really wouldn't mind making an average salary doing lab tech work for a while until i want to go back to school.
 >> Fuck Nunkingold - Sat, 18 Oct 2014 23:50:15 EST ID:f5OSHUfT Ignore Report Quick Reply >>75576>. but i've been told those are just as bad as BS. getting a PhD and going into academia after isn't something i ever planned to doTrust me there's a lot more options for a masters. In some ways, it's even better than a phd since you don't spend 5 years getting paid shit and being in academia.> i really wouldn't mind making an average salary doing lab tech workThen get a masters, because that's what it's good for. You don't need a phd. You might get lucky, and be fine with a BS. Or, you can be pretty secure with a masters.Like I said, you might want to consider it as a backup. I really really wish I did and got those fucking stupid letters.
 >> Clara Dondlestone - Sun, 19 Oct 2014 00:21:53 EST ID:V5qF06Hs Ignore Report Quick Reply >>75577the only reason i don't like the idea of a masters is you have to pay a dick ton more in tuition, whereas you get paid as a phd. i consider the tuition waiver to be a fair exchange for a decent salary for the time in classes. but i just do not want to do that if i don't have to, man...can you explain more about the letters?my current PI and my previous boss (worked as a convenience store clerk) are the only 'professional references' i have, though i'd only put my current PI on a job app for something related to biochem.where would i find other people to vouch for me if i've only worked for one person? would that be something to do with the lab rotations that grad students do? the first person just left last friday and we're getting a new person soon.would i need to get my master's just to do that? surely i could just hop around a few different labs on my PI's recommendation as a good lab tech?
 >> Fuck Nunkingold - Sun, 19 Oct 2014 01:15:17 EST ID:f5OSHUfT Ignore Report Quick Reply >>75578>the only reason i don't like the idea of a masters is you have to pay a dick ton more in tuition, Oh. At my uni, they paid for masters too. I understand not wanting to do it then.I shouldn't be giving advice, since I don't know how to get employed myself.> surely i could just hop around a few different labs on my PI's recommendation as a good lab tech? That's what I thought. It didn't work like that for me, despite having a glowing recommendation and a pretty broad skillset.I've heard "networking" is the key. So do that, whatever it is.

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