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Oxycodone Reactions Summed up Neatly by Bombastus !lnkYxlAbaw!!7zlcjO/U - Sun, 24 Jan 2016 16:04:57 EST ID:V1Ngvki3 No.77560 Ignore Report Reply Quick Reply
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Okay, so we had a failure with the reactions of oxycodone with a fucked up friend of mine a while back. He had given me some failed hydrogenated species of 14-hydroxycodeinone which was one of the weirdest highs I have ever experienced in my life. I think I took around 25mg of it.
I think that was a weird kappa-agonist with some mu-effects.

Regardless, a few things that make the last thread obsolete are as follows:
  1. 14-hydroxylation of any morphinan requires the double bond at the 7th position for the enol to occur. Otherwise, the oxyacid cannot form the tertiary alcohol due to lack of reactivity.
  2. Acetic protection group seems to be the easiest way to create 14-hydroxy on the morphinan groups. It has the most yield if not starting from thebaine. The next best thing would be an annoying Cobalt (III) salt which I never knew existed. It seems like this unstable salt can be used as a catalyst for codeinone > hydroxycodeinone. But it's really annoying to make.
With no protection, the yield is an atrocious 20%. With the protection, you can get the yield up to 80%.
3. Almost all side reaction waste products are water soluble after basifying. Unless you're unlucky, there is little requirement for columns during these reactions.
4. You cannot avoid hydrogenation if making oxy from cod.

In summary, unless you want to follow the method step by step on the erwoid page that summarises Codeine > Oxycodone, you won't produce nearly the same results with the available chemicals.
With no protection, you could easily achieve a 20% yield of oxycodone from codeine.

He's also currently working on a more efficient method to oxidise codeine to codeinone (hopefully using bleach and vinegar), a more efficient method to hydrogenate, as well as a better peroxy-acid to put the 14-hydroxy group on.

Pic related. Artefacts. We need those 1900 German artefacts that tell us how to morphine.
12 posts omitted. Click Reply to view.
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Cornelius Buzzbanks - Sun, 21 Feb 2016 17:34:34 EST ID:p0QLwGpF No.77684 Ignore Report Quick Reply
>>77676
It's possible that the reaction creates oripavine. Which has to be extracted to purify the hydromorphone
>>
Cornelius Buzzbanks - Sun, 21 Feb 2016 17:53:58 EST ID:p0QLwGpF No.77685 Ignore Report Quick Reply
Bombastus have you tried that the noble metal catalyst synthesis of hydromorphone?
>>
Bombastus !lnkYxlAbaw!!7zlcjO/U - Mon, 22 Feb 2016 00:44:30 EST ID:sqhxA+9X No.77686 Ignore Report Quick Reply
>>77685
Yes and it turned me gay. Successful I'd say might do it again one day.
>>
Ian Begglestone - Mon, 22 Feb 2016 15:47:37 EST ID:VuOwbgQK No.77687 Ignore Report Quick Reply
>>77686
Ok if your really gay then where did you hide your gay gold?
>>
Henry Pombleson - Tue, 23 Feb 2016 14:20:24 EST ID:uPn+UTf4 No.77689 Ignore Report Quick Reply
>>77686
How did u purify that shit from the rest of the chemicals lmao


VM&P Naphtha by Molly Bannerlock - Wed, 18 Nov 2015 12:15:21 EST ID:u4vCvpNb No.77376 Ignore Report Reply Quick Reply
File: 1447866921478.jpg -(91226B / 89.09KB, 720x900) Thumbnail displayed, click image for full size. 91226
What exactly is VM&P Naphtha? You most likely know it's used for the extraction of DMT, but it's not really accessible if you don't live in America.
Some guides say you can use basic paint thinner. Is that true?
17 posts and 5 images omitted. Click Reply to view.
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Vehk !7HYGxe5v5c - Mon, 15 Feb 2016 16:40:26 EST ID:PPkNLWzw No.77661 Ignore Report Quick Reply
>>77657

> secondary school

Are you Irish, fam?
>>
Charles Bardforth - Sun, 21 Feb 2016 07:17:05 EST ID:TtNn9wfR No.77681 Ignore Report Quick Reply
>>77661
Dutch.

I used to write down middleschool (that's what all secondary education is called in the Netherlands - Middelbare School) but I was tired of getting banned for being socalled "underage".

Since Dutch "middleschool" can be 6 years long (if you take the "A only class" courses) , and I had to redo a year once, I was able to legally browse 420chan during the last two years of "middleschool".
>>
Ebenezer Sunderwill - Sun, 21 Feb 2016 13:48:38 EST ID:cKZnkWSj No.77682 Ignore Report Quick Reply
>>77681
Those Dutch Madrasas can't possibly be any good at teaching science, I thought that all European schools tought was pansy social awareness crap?
>>
Bombastus !lnkYxlAbaw!!7zlcjO/U - Sun, 21 Feb 2016 15:57:33 EST ID:Ko/zFYye No.77683 Ignore Report Quick Reply
Hahaha I huff paint thinner
>>
Phyllis Moblingbodging - Wed, 02 Mar 2016 14:01:20 EST ID:TtNn9wfR No.77702 Ignore Report Quick Reply
>>77682
Go fucking kill yourself you fucking the future cocksucker.


New, Novel, Potent Opioids by Bombastus !lnkYxlAbaw!!7zlcjO/U - Thu, 04 Feb 2016 17:39:55 EST ID:ElYFdcKO No.77619 Ignore Report Reply Quick Reply
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Thants (our br/opi/ in Canada) has recently nodded off W-18, which is a totally novel opioids that hasn't seen street time. So /chem/ might as well talk about it.

The current method of synthesis is outlined in patent no. US4468403
>A mixture of 1-phenylethylpiperdylidene-2-(4-chlorophenyl)sulfonamide (1.17 g, 3.11 mmol) W-15, 90% fuming nitric acid (3.0 ml) and concentrated nitric acid (2.0 ml) were stirred vigorously for 4 hours at 25° C.
Preparation of 1-phenylethylpiperdylidene-2-(4-chlorophenyl)sulfonamide is not outlined, unfortunately. But it's pretty easy to figure it out as it's a straight chained molecule.

So uhh ya. W-18. Talk about it. It's legal everywhere except in Sweden right now. I guess that is a thing. It was discovered by Knaus back in the 80s who was a pretty sick guy who liked opioids. He's had a lot of work doing shit like that. Here's some condensed reading:
http://chemistry.mdma.ch/synthforum/about635.html

W-18 is the 18th item on his patent list: http://www.google.com/patents/US4468403
It includes up to 35 different, unscheduled, and very potent, novel opioids that do not rely on the morphinan group. Check em.
9 posts and 1 images omitted. Click Reply to view.
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Fanny Bardham - Wed, 10 Feb 2016 18:20:53 EST ID:DIZp74tV No.77648 Ignore Report Quick Reply
>>77641
Thanks for the info on pipette tolerances. Someone doing careful weighing and serial dilution might be interested in a microliter syringe also.
>>
Bombastus !lnkYxlAbaw!!7zlcjO/U - Sun, 14 Feb 2016 17:21:18 EST ID:vbS8CtHn No.77660 Ignore Report Quick Reply
>>77646
Ingested, yes. I would not recommend IVing anything that requires volumetric dosing (serial dilution). From what I hear on /opi/, fentanyl and all their analogues as well as other super potent opioids do not have an appreciable nod.
Their oral BAs are all high enough as they don't have metabolizable groups like the conventional morphinan opioids do.

I mean, if you really want to IV, you could just put the 100ml of final solution into a pressure cooker and sterilize it that way for injection every time. But the reality is that you still should not be injecting any random ass powder off the internet.
>>
Emma Turveyford - Sat, 20 Feb 2016 12:52:53 EST ID:p0QLwGpF No.77669 Ignore Report Quick Reply
Hi bombastus sorry to bother but did your oxycodone synthesis tek work?
>>
Bombastus !lnkYxlAbaw!!7zlcjO/U - Sat, 20 Feb 2016 15:03:46 EST ID:4ppVjZXo No.77672 Ignore Report Quick Reply
>>77669
a few friends of mine and me got together a few years ago and made something like 5g and split it evenly. That was a sweet adventure.
That binge and subsequent withdrawals made them almost all give up opioids after that. Which tk did you talk about?
>>
Lydia Picklock - Thu, 07 Apr 2016 17:59:34 EST ID:IsDQAl0D No.77814 Ignore Report Quick Reply
>>77619


okay well i have experience with butyrfent. all i have to say is be prepared to poke yourself full of holes if IVing. the highs are so cracky that they will have you compulsively redosing every few hours, effectively turning you into a pin cushion until you flush the sheet.


And that person was me by Henry Bleshdock - Thu, 18 Feb 2016 20:25:38 EST ID:RC13Ktoh No.77668 Ignore Report Reply Quick Reply
File: 1455845138359.jpg -(5910B / 5.77KB, 225x225) Thumbnail displayed, click image for full size. 5910
High school chemistry class, we were working on a lab where we had to make little squares on a piece of plastic to perform tests in, and the squares had to have 2 cm long sides.

This kid at my lab table, who had already proven to be not so bright in class many times beforehand, got our teacher's attention, then asked what a centimeter was.

After the teacher thoroughly explained what a centimeter is, and where exactly to find them on the ruler in front of him, the student turned to us and said:

"I'm still not understanding this centimeter thing."


Water Bottle Plastic Heat Danger? by Hedda Drongernon - Tue, 16 Feb 2016 20:31:21 EST ID:RLSN+6eM No.77663 Ignore Report Reply Quick Reply
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You know when you leave a plastic water bottle in your car and it gets hot for hours and then when you drink it you can taste plastic?
So where is that plastic taste coming from? And if it does mean that there's plastic chems, then how dangerous is it?
>>
Hedda Drongernon - Tue, 16 Feb 2016 20:47:54 EST ID:RLSN+6eM No.77665 Ignore Report Quick Reply
>>77664
And how much of this post is satire?
>>
Bombastus !lnkYxlAbaw!!7zlcjO/U - Thu, 18 Feb 2016 01:51:05 EST ID:wvu8YcUF No.77666 Ignore Report Quick Reply
>>77665
>wat post
question answered
>mfw
see OP's photo
>>
Bombastus !lnkYxlAbaw!!7zlcjO/U - Thu, 18 Feb 2016 01:55:23 EST ID:wvu8YcUF No.77667 Ignore Report Quick Reply
>>77663
Polyethylene terephthalate can break down to form Bisphenol.
That's probably the taste and can fuck with your hormone system. Don't drink the water if the water bottle has touched hot water, just a generally bad idea.

It's not super toxic. But don't do it.


Considering grad school by Cornelius Wosslestack - Sat, 29 Aug 2015 02:41:37 EST ID:A/ZPVECK No.77050 Ignore Report Reply Quick Reply
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So I'm considering getting a master's in chem. I have always enjoyed chemistry, but I majored in liberal arts. That being said I recall a fair amount about it from taking AP chem in high school.

In any case, I'd mainly like to focus on materials science because I have some experience with machining and fabrication. I'm also thinking I'd end up in education, because I like teaching and that seems like a solid way to go in terms of job security and salary.

Really I'm just looking for advice here. I love chemistry, but I'm pretty damn rusty and I have no real college experience - AP chem in high school is generally a lot easier than an actual college course.

First steps? Things to consider when looking at programs? Any and all tips or advice would be appreciated.
4 posts and 1 images omitted. Click Reply to view.
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Vehk !7HYGxe5v5c - Mon, 31 Aug 2015 14:51:54 EST ID:9sH3Ao2e No.77091 Ignore Report Quick Reply
>>77090

I'm an undergrad Biochem student in southern Ireland. We have two main pharmaceutical hubs here, one in Dublin and one in Cork. The pay is slightly higher in general in Cork. Employment is plentiful as there are many different companies and they are frequently hiring (in fact, something like 20% of the lab technicians at a Pfizer lab I interned at didn't even have a B,sc. Although most of these people are dinosaurs who started working before hiring was standardized).

As a lab technician, you are looking at 30 - 35k starting, which is 40k dollars. If you manage to get a full QC position (helpful if you have your degree in or are spec'd in analytical chemistry) you could see that pushing 40k euro, though the work is quite banal and repetitive. Other facilities do biotech rather than organic synthesis and these would be a bit more stimulating. These places are harder to get hired in though, it's the Pharm-Org-Analytical jobs that are plentiful. If you are good there is also plenty of room for upward mobility in QC/QA labs.

By the way, why are you getting a master's instead of a Ph,D? Most research fellowships (these often pay quite nicely) require Ph,D minimum, and it's also what you need to get a job in basic research in industry.

As I was saying, the market is very comfy here, but work experience is kind of important - most of our colleges here do placements to pad CVs. If you want to work/live in Ireland, it is possible to get dual-citizenship if you have Irish ancestry and are an American citizen.

Personally I am doing this course as a pre-med to become eligible for graduate entry to Medicine, but it is very reassuring to know I can always fall back to industry if my aspirations in Medicine or Research don't work out.
>>
Edward Pecklewill - Tue, 01 Sep 2015 22:26:08 EST ID:uGD5aNS6 No.77102 Ignore Report Quick Reply
>>77091
>If you want to work/live in Ireland, it is possible to get dual-citizenship if you have Irish ancestry and are an American citizen.
Huh. Do you know anything about Ireland's biomedical research? I wanted to do a post doc abroad, was considering centeral Europe/Germany if I can find a position. Never really considered Ireland, even though by ancestery I am primarily Irish.
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Vehk !7HYGxe5v5c - Wed, 02 Sep 2015 13:05:09 EST ID:ILVV3idO No.77105 Ignore Report Quick Reply
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>>77102

Ireland has a strong history of medical and pharmaceutical research, particularly in oncology. It mostly centers around the main national universities.

http://www.ucd.ie/research/health/
https://www.ucc.ie/en/research/overview/
>>
David Honeybury - Tue, 08 Sep 2015 18:40:45 EST ID:dkMoIz4p No.77130 Ignore Report Quick Reply
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>>77050
I mean you could take some more classes. College chemistry is nothing like high school though. Basically you've taken at most two semesters of college chemistry. You'll basically have another degree.
I think to be a high school/ junior high teacher you could get by with maybe about gen chem (2) + o chem (2) + p chem (1-2) + inorganic + quantitative analysis + math + physics (2). This is only the minimum possible requirements of course (I'm sure most colleges will require much more). So, not to dissuade you but, there would be quite a few classes that you would need to take first even for a bachelors degree. On the plus side, you'll have all of you're generally classes covered.

As far as master programs go, you're adviser will end up mattering a lot more than the college will. Choosing a good adviser who is well known in the field and is easy to get along is what you're looking for.

Anyway, worth pointing out that chemical engineers have good job prospects and will probably end up taking quite a few of the same courses.
>>
A Wizard - Wed, 09 Sep 2015 01:41:46 EST ID:eg2eHljf No.77133 Ignore Report Quick Reply
>>77130

What? That's not how you pick your master. You find a complete asshole who knows his shit and hates his students, and you make that motherfucker laugh every damned moment you get the chance, except the times he's trying to focus.

The reward is that he teaches you, at the expense of the annoying dipshits you compete with. He'll probably even show you things he shouldn't too, just in hopes you use it.


The biological singularity by Sophie Piblingforth - Thu, 04 Feb 2016 19:54:08 EST ID:3xOkFk4I No.77623 Ignore Report Reply Quick Reply
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the technological singularity is discussed and well known enough but wouldent the same assumptions more obviously lead to a biological one (assuming we are around after the former). the tech one is basically the unknown after we create a computer that can create a better computer than we can. however a computer (unless we specifically design it to) is not necessarily interested in procreation or its own survival. a biologically altered human though would probably still have those drives in tact (unless we remove it). not that an AI cant value itself and wish to spread or a modified human couldent do the opposite its just that its less likely. both are likely to be started by us but probably completely with very different motives. a biological organism -such as ourselves- is designed by evolution to spread and we may consciously remove that whereas an AI could develop it but it isnt an integral part of its original form. we are moving to a point where we can program genes almost as easily as we code an AI thus the exponential improvement in ability is just as feasible biologically as digitally. consciousness dosent automatically lead to a desire to continue; the childless humans (and more so the suicidal) have evidence to share on this. why should an intelligence, without an inbuilt need for survival and legacy, necessarily adopt both?
fundamentally wed be in the same boat as with machines in hoping they dont turn against their creators but in practice we are more likely to empower and pass on our need to continue to the latter. hell, we might even be cheering it on; it would be much easier for us to embrace designer babies than really advanced software.

tl;dr: we will soon be able to make computers that can make better computers than us. after a slight time lag we will be able to create humans that cant create better humans than us. the former will have the advantage of being earlier but the latter is more likely to be inherently interested in advantage and promoting the same.
not that its even automatically a bad thing since our creations will exceed us (by definition in this case) and will be able to do more than us. they probably wont automatically (see what i did there?) feel a need to turn on us and …
Comment too long. Click here to view the full text.
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Hugh Battinggold - Mon, 08 Feb 2016 11:38:47 EST ID:FHFwCltH No.77636 Ignore Report Quick Reply
bump for interesting post
>>
David Blapperridge - Thu, 11 Feb 2016 13:44:59 EST ID:cIUKn2oY No.77649 Ignore Report Quick Reply
The danger of malicious AI doesn't lie in the Matrix type robot rebellion tho. It's more about whether AIs will fully understand the implications of their actions in the same ways we do. Unless you simulate a human brain, AI won't ever be human. It will be something else entirely.

For example, lets say we program an AI to protect and take care of us. The AI's prime directive is to keep humans safe, so true to it's programming it traps all us in a never ending stasis. It has our best interests at heart, yet it effectively ended humanity. Or you make an AI to perfect some product, and it decides to use the entire planet for computational substrate in order to do so.

Kind of a cop-out, but you get the point right?
>>
Sidney Fanworth - Fri, 12 Feb 2016 03:53:13 EST ID:X6HIP3d/ No.77650 Ignore Report Quick Reply
>>77649
No. In fact I think you are intentionally shilling misinformation
>>
Nigger Mucklepune - Sat, 13 Feb 2016 06:26:29 EST ID:+DZfgoAX No.77656 Ignore Report Quick Reply
>>77649
That's essentially the premise of the grey goo scenario which was dreamed up by Prince Charles. It's plausible but you'd have to fuck some shit up badly to have it happen. Bear in mind that for it to occur you have to have that sort of tech in the hands of fuckwits, and if fuckwits can get it, then there's more powerful technology in the hands of other people who are more competent and aware of the dangers.

I get your point but you're talking about design faults. People will test and experiment and build failsafes on anything that's high risk enough and only roll out something that can end life as we know it if they already have a way to stop it.

Anyway I think that it's also quite possible we'll merge with the machines. Superhuman humans will just be a stopgap or an option for people unwilling to become a sentient nanobot swam or join the network.


Graphing Woes by Hannah Fuckinghood - Wed, 03 Feb 2016 14:21:25 EST ID:bATSxV63 No.77615 Ignore Report Reply Quick Reply
File: 4.jpg -(80085B / 78.21KB, 666x69) Thumbnail displayed, click image for full size. 80085
If you have a graph where the different series represent different units (e.g. conecentration, pH, and a Ratio), what should you label the Y-axis?

In return, bountiful tits!

Mods, sorry if this meant to be SFW board. Just sandwhich my picture, if you could make it a delicious turkey sub that'd be swell
>>
Fuck Mobblepadge - Thu, 04 Feb 2016 05:07:15 EST ID:NyZzVSs3 No.77616 Ignore Report Quick Reply
>>77615
Personally I'd put them all on a 10 scale and just use that
Label wise I'd be a link to the math of each line so i could work my way back to the orginal value if need be
>>
Bombastus !lnkYxlAbaw!!7zlcjO/U - Thu, 04 Feb 2016 17:35:26 EST ID:ElYFdcKO No.77618 Ignore Report Quick Reply
>>77615
Work safe board pls. At work. Not safe.

RIP in fucking pieces.
>>
Archie Conninghood - Tue, 09 Feb 2016 10:27:17 EST ID:bATSxV63 No.77640 Ignore Report Quick Reply
>>77616
I'm guessing you dont meant a log scale, just a 1 - 10 type dealie?
Seems like a good idea, i'll give that a playaround in excel.
Also, if I on the X axis I was measuring 4 different pH values, it would seem to make sense to just label them via the tube numbers.
However, apparently that is not acceptable for the report i'm writing.
I can't exactly label the X axis as pH either, as i've already got values for that on the Y axis and the Series.
Any advice would be much appreciated.
>>
press !QUHukXEvkY - Tue, 09 Feb 2016 12:41:05 EST ID:MD/oThse No.77642 Ignore Report Quick Reply
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>>77640
in excel you can use atleast two different y-scales.

and im guessing that youre plotting a titration?
in that case id say that the pH has to be in the y because it depends on the volume of added solution

could you just give us a brief run down on what your experiment is?
>>
Archie Conninghood - Tue, 09 Feb 2016 13:12:15 EST ID:bATSxV63 No.77645 Ignore Report Quick Reply
>>77642
Of course! I'm sorry, I should've led my post with that.
I'm writing a report for an experiment I did in my Intro To Pharmacology class.
The experiment is, "The Uptake Of Salicyate Into Yeast Cells".
It's to demonstrate the phenomena of the passage of weak acids and bases between lipid membranes.
The report centers on the manipulation of the Henderson-Hasselbalch formulae in order to determine the concentration of salt [A-] within the yeast cell.
I've managed to create a graph of the standard curve with no trouble (R2 of 0.9989, very pleased about that), but the last graph showing the relationship between pH, [Salicylate] and [Salt] (both inside the cell and the supernatant) is proving trickier.


Uni decisions by Charlotte Clellerstire - Thu, 21 Jan 2016 22:52:57 EST ID:YFGLWvGz No.77548 Ignore Report Reply Quick Reply
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Need some advice. Tossing up between continuing my bachelor of science (only completed 1 semester) or changing to a bachelor of agriculture which is aimed more at what I want to do (Agronomy) I feel as though I wouldn't get an agricultural job with a bachelor of science. And should I travel to a prestigious University to study? or study at the University in my own home town. Any advice is much appreciated as I am having a hard time with these decisions and 3 years is a big commitment and I dont want to finish one then look back wishing I chose the other one. I am Australian if that matters. Thanks
7 posts omitted. Click Reply to view.
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Vehk !7HYGxe5v5c - Thu, 28 Jan 2016 11:08:55 EST ID:VhvSklsj No.77586 Ignore Report Quick Reply
>>77572

Second, it's important that we get him off /med/ where his baloney could have more serious coneequences as well.
>>
Phineas Fengerdale - Fri, 29 Jan 2016 11:25:26 EST ID:GgTTTkur No.77596 Ignore Report Quick Reply
>>77586
Yeah like getting me to eat dirt for a head ache!
>>
Vehk !7HYGxe5v5c - Wed, 03 Feb 2016 03:58:54 EST ID:/ZLfJIXn No.77613 Ignore Report Quick Reply
>>77608

Yeah, because everytime the mods locked one of your shitpost threads or banned you from /lit/ they forgot to check the IP, it's all a a big conspiracy. It's obvious what you're doing and it's pathetic.
>>
Bombastus !lnkYxlAbaw!!7zlcjO/U - Thu, 04 Feb 2016 19:05:09 EST ID:ElYFdcKO No.77621 Ignore Report Quick Reply
>>77596
LMFAO. EHUEHUEHUEHUE. Please screencap that or yellowtext the story.

>>77585
In biology, biological cultural assistant, biology consultant, office data entry job, teacher
In chemistry, simple laboratory assistant, anal. org. etc. Oil. Pharma
In physics/math, any financial job, teacher.
those are just what i can think of within 15 seconds.
>>
Shitting Pishkare - Mon, 08 Feb 2016 14:54:18 EST ID:5RCKIYnW No.77637 Ignore Report Quick Reply
>>77585

Any job you can do plus alot more.


wheresmymotherfucking biology board by William Gapperbat - Thu, 31 Dec 2015 22:24:52 EST ID:IaY1gFOQ No.77484 Ignore Report Reply Quick Reply
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hey, fuckin, if i run a current through some shit, like a strong fuckin "im gonna connect extremely powerful device A to mains outlet but the wire's going through thistank of water

will it kill everything? even the microbes? even the tiny superhero antman?
1 posts omitted. Click Reply to view.
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James Chommerbury - Tue, 05 Jan 2016 00:51:38 EST ID:GEEvdbi2 No.77498 Ignore Report Quick Reply
Hopefully it only kills you
>>
Ebenezer Sonkinman - Wed, 03 Feb 2016 13:24:04 EST ID:yvLCKMRO No.77614 Ignore Report Quick Reply
The OP is stupid, the question is not. Does anyone actually know whether microbial life can survive high voltage?
>>
Sophie Chonkinchetch - Thu, 04 Feb 2016 11:53:58 EST ID:wE3/tzia No.77617 Ignore Report Quick Reply
>>77614
  1. Inoculate appropriate (liquid) culture medium with some non-pathogenic, non-sporulating bug.
  2. Split the broth between two sterile electrolytic cells (to rule out the influence of the electrodes or whatever).
  3. Apply suitably high voltage to one of the cell, for a suitable amount of time.
  4. Wait for the bugs to multiply, then compare optical density.
  5. Repeat.
  6. Report.
>>
Clara Dripperdale - Sun, 07 Feb 2016 21:42:53 EST ID:+8mgBrQ3 No.77634 Ignore Report Quick Reply
/Dino/
>>
Hugh Battinggold - Mon, 08 Feb 2016 01:10:15 EST ID:FHFwCltH No.77635 Ignore Report Quick Reply
Presumably it would eventually kill microbes.
One common means of transforming (aka introducing plasmids) into bacteria is elctroporation, which is essentially zapping bacteria with pulses of electricity. These pulses generate small pores in their membranes, into which plasmid DNA slips in.

However this is done in a highly controlled setting, high intensity bursts, and even that produces holes in their membranes, and even that kills alot of the bacteria. Its assumed that even if only 1% survives, in a culture of >1million cells thats no big deal, you still have 10000 cells that will produce viable colonies.

Now, after that little tangent, electric currents are used as a form of chromotography known as electrophoresis. Essentially when you run DNA or protein gels you separate by size based on charge. Since most molecules in the cell are either negative or positively charged, you would essentially tear the cell apart based on the charge of molecules within it. Even the membrane would be torn apart as the negatively charged head groups are pulled toward the cathode, with basic proteins pulled toward the anode.


UNCLE FESTERN BOOKS by Hugh Geddledale - Fri, 08 Jan 2016 21:26:41 EST ID:gtW0pF7l No.77510 Ignore Report Reply Quick Reply
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hello there /chem/ I've been reading uncle festern books and I would like to ask:

is this serious chemistry to be applied? what do you think of his methods? good, bad or dangerous?

thanks, SLAYER to all chems (seeing Bombastus still here is great as fuck, I've been outta here for long time)
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press !QUHukXEvkY - Fri, 22 Jan 2016 09:48:17 EST ID:trptNJfh No.77549 Ignore Report Quick Reply
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>>77546
HI/red P is nice for intermediate knowledge of chemistry and lab techniques
things like P2P condensation with in situ prepared methylamine reduced over Al/Hg or Grignard reactions for amphetamine are a whole other story. for the more advanced methods you either need huge personal funds, sneaky access to a uni lab or a criminal background

seeing as you dont have any background in chemistry, you should try to opt for the safest methods whenever possible. and id recommend looking up chemistry write ups for practical lab courses in OC1, these often have extensive risk assesment and security information. just to get a feel for proper chemistry and how to take pride in your work

dont be one of those shake and bake fuckers that leave bombs laying around in the forrest, because if there happens to be even a single circle of hell, those fuckers belong there.
nb
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Alice Trotwill - Thu, 28 Jan 2016 21:02:14 EST ID:t0ksP9bJ No.77587 Ignore Report Quick Reply
>>77583
Haha I like wordle too
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Phineas Fengerdale - Fri, 29 Jan 2016 03:00:45 EST ID:GgTTTkur No.77593 Ignore Report Quick Reply
>>77587
I don't like to talk in nouns
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Phyllis Wuckleford - Sun, 07 Feb 2016 14:48:34 EST ID:ig6EEMwa No.77629 Ignore Report Quick Reply
>>77549
Shake and bake is useful because you can burn the evidence and it's almost if not free.
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press !QUHukXEvkY - Tue, 09 Feb 2016 12:44:51 EST ID:MD/oThse No.77644 Ignore Report Quick Reply
>>77629
yes, just burn the evidence. while were at it, burn the forrest down, just to make sure there are no witnesses
then continue with self immolation, that way you wont fail any urine tests


Overwhelming amount of physics by Studying tips, tricks, advice, and general stories - Wed, 21 Oct 2015 20:39:25 EST ID:Fd+bN9n4 No.77312 Ignore Report Reply Quick Reply
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Student here, I'm taking a calculus based physics course and it's ruining my life. Literally spend the whole day, roughly 6-8 hours working on physics problems daily because of the overwhelming amount of work that goes into our grade. To the point that I have been neglecting my other subjects and my grades in those classes are slowly going down, except physics.

My question is, is there any tips or advice on how to juggle such intensive courses and exceed in all of them at the same time? I need to reevaluate my plan because I feel like if I spend less time on physics, my grade will go down since the material is so dense.

Stories of people in higher academia are appreciated. Also tips to unwind and not let physics ruin your life.

For example, I worked on 8 hours of crunching problems in my problem set all day. Now that I'm home, I feel the need to continue but my brain feels like mush.

PS.

I take adderall on the weekday and I don't have ADHD, just because I want to pass this class.
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name - Tue, 05 Jan 2016 01:33:01 EST ID:97gqDNib No.77500 Ignore Report Quick Reply
just graduated with a degree in astrophysics from UC santa cruz. I got a C my first semester of physics at community college. You're fine.

P.S. screw mechanics
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Samuel Blatherson - Thu, 21 Jan 2016 18:49:25 EST ID:Qhw9/k12 No.77547 Ignore Report Quick Reply
>>77312
Classes like these are built to be extremely difficult and made to be frustrating and shitty, anon. Don't put too much of your personal worth into getting great grades in classes like this, just try to get a mastery of the material and work forward from there. Meet with your professor and go to the help room. 6-8 hours a day reeks of inefficiency, anon. Get some help, this stuff is a bitch to teach to yourself.
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Doris Sagglehood - Sat, 23 Jan 2016 13:41:40 EST ID:Hh8MxSMi No.77554 Ignore Report Quick Reply
>>77500
Heh, yeah. Same here. I took my first semester of physics for engineers over a 6 week summer semester.

Got a C, but hey that's all I needed. Don't even trip, dawg(OP)
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Hamilton Ganninghere - Tue, 26 Jan 2016 10:52:54 EST ID:W06/MA+o No.77568 Ignore Report Quick Reply
Double Math & Physics major chiming in. There's no shortcut. You signed up for 8 hours of work a day so what do you expect?

I don't even have access to adderall despite having mind autism. Shit sucks.
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Nigger Blinkinwater - Wed, 27 Jan 2016 00:35:11 EST ID:KI/L6/gT No.77580 Ignore Report Quick Reply
>>77568
Dude that's what like certain groups would likely to support certain things


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