AnonAccount: What is it, and what does it do? - Q&A Thread
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Cannabis Extract for E-Cigarette/Vape-pen (rate / critique / input?) by Ernest Drinderville - Sat, 17 May 2014 17:19:46 EST ID:fW8Ohdad No.74729 Ignore Report Reply Quick Reply
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1.) In a beaker, thoroughly dissolve ~2g of high-potency hash oil or cannabis wax (ideally 70% cannabanoid or greater) into 50ml of 90% or greater Isopropyl alcohol.

2.) Strain the mixture through cheesecloth or very fine mesh to remove impurities from oil or hashish.

3.) Take the strained mixture and heat it to 81°C and stir, allowing the alcohol to evaporate (this is important because this type of alcohol is harmful to humans; you don't want to leave any behind!), just leaving behind a pure cannabanoid extract.

4.) Mix this extract with 30ml of vegetable glycerine and 20ml of propylene glycol. Stir until thoroughly mixed throughout. (OPTIONAL: Add a little peppermint oil or your favorite flavoring to taste.)

5.) Put in your favorite vape and enjoy!
3 posts omitted. Click Reply to view.
Walter Honningsterk - Mon, 19 May 2014 14:06:46 EST ID:72wOh3Tw No.74738 Ignore Report Quick Reply
>nor smells like cannabis

Good luck with that.
Cornelius Cabblewell - Mon, 19 May 2014 15:43:46 EST ID:T5wS+R1s No.74739 Ignore Report Quick Reply
>get BHO
>get a dab vape pen

I've hit this thing in movie theaters. Anything else is just retarded
Ebenezer Broddleshit - Fri, 23 May 2014 19:41:21 EST ID:H2YQ+emU No.74753 Ignore Report Quick Reply
op have you actually done this
if so post pictures
also approximatley how many hits until you get high?
Jack Pillerstone - Sun, 08 Jun 2014 22:30:22 EST ID:JT/zeY14 No.74814 Ignore Report Quick Reply

Steps one through three are just going to remove impurities that are not soluble in alcohol but those were likely already removed during the manufacture of hash oil or wax since the butane, co2, and alcohol extraction methods are most commonly used. Only cold water extraction would leave behind alcohol insoluble impurities. I have dissolved extract in vape solution but the boiling point is well below the temp at which thca decarboxylates. I would replace the first three steps with a decarboxylation step before dissolution in vape solution.
George Cinnergold - Thu, 12 Jun 2014 13:22:51 EST ID:ssuZZR2V No.74833 Ignore Report Quick Reply
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Blood Test Results by Hugh Toothood - Tue, 20 May 2014 07:31:44 EST ID:87avDrkn No.74740 Ignore Report Reply Quick Reply
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Posted this in /med/ but feel maybe people with a scientific background can help a lot here if possible.

Basically I had to go to hospital and as a result needed a blood test, the results came back (in their words) as me having very excellent blood apart from low levels of magnesium which is normal among many people anyway. However all of the stats I barely understand and do not know what figures are good or bad etc, can anybody with the relevant knowledge explain them to me and say if they are ok?
1 posts omitted. Click Reply to view.
Thomas Dobberlock - Tue, 20 May 2014 16:55:30 EST ID:ACfRRiH3 No.74745 Ignore Report Quick Reply
Thanks for the reply, basically I am not exactly worried about something in particular, I was pretty out of it at the hospital and didn't speak much about the results. Now I am more coherent I am just curious to know what each statistic means. By the way what do you mean by a subclinical infection?
Edward Mangerwill - Thu, 22 May 2014 08:41:17 EST ID:Mj7GCD93 No.74746 Ignore Report Quick Reply
'subclinical infection' sounds like your immune system is working on something,hospitals are actually the #1 place to pick up infection
but your numbers look good
Ian Passlefet - Mon, 02 Jun 2014 17:28:10 EST ID:im1yYESs No.74773 Ignore Report Quick Reply
high monocite

it's lupus
Eliza Nellersut - Tue, 03 Jun 2014 23:39:25 EST ID:oRLfV4+h No.74780 Ignore Report Quick Reply
Damn dude, your platelets are high.
Its probably anal prolapse, I'd get that checked
Simon Sappernedging - Sun, 08 Jun 2014 02:04:51 EST ID:DyzoY98k No.74804 Ignore Report Quick Reply
Creatinine and eGFR is used to look at kidney function.
eGFR is essentially how much is being flowed through your kidneys.
Creatinine is a basic indicator to see if your kidneys are filtering properly. ie if you have a shitton of creatinine your kidneys aren't working

Sodium, Potassium, and Chloride are all electrolytes.
CO2 can be used to show the acidity of blood and lung function.
Calcium and phosphorus (phosphate) all have to do with bones. Their levels depend on each other and they can be detected in blood and determine kidney and parathyroid function.
Magnesium is another electrolyte needed for muscle function.

WBC are used for your immune system. Too many means you have an infection.
RBC are used for respiration.
All those three letter acronyms are all physical characteristics of your blood. How much hemoglobin you have, how concentrated it is, RBC volume, etc.
Platelets are so you don't bleed out all the time.
They do a smear to see how your blood looks to back up all of that information.
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Grow your own drug? by Esther Bezzlehid - Wed, 23 Apr 2014 23:03:20 EST ID:SneqK8/u No.74586 Ignore Report Reply Quick Reply
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Why do you think no one has tried to grow their own coca/poppies indoors? Just surprised since weed and shrooms have been cultivated indoors for quite a while now. Granted processing cocaine and (probably) heroine takes more work but still, hydroponics have become complex. So, I would reason that drug extraction from other plants doesn't seem too difficult for some of the more curious in the age of the internet.
>Anyway, why do you think this hasn't been tried yet?
8 posts omitted. Click Reply to view.
Molly Bugglefield - Fri, 25 Apr 2014 19:43:13 EST ID:ZXl1qmAy No.74619 Ignore Report Quick Reply
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except coca tea does not get you high and it tastes like grass clippings (it is good for headaches/altitude sickness though)

ya gotta do like the peruvian grandmas do and chew all day erry day
Martha Duckgold - Wed, 14 May 2014 18:16:07 EST ID:fkvV135M No.74712 Ignore Report Quick Reply
Coca takes a very long time to mature as well.
Hedda Dommlestock - Thu, 15 May 2014 16:01:11 EST ID:T5wS+R1s No.74716 Ignore Report Quick Reply
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I have spent the last few years researching exactly this.

a lot of synthetic drugs can also be made this way too.. Methamphetamine would not exist without our good friend Ephedra Sinica. MDMA, LSD all come from plants.

Unless you live in South America or somewhere with similar climate I don't think you will have much chance growing coca. maybe if you had a totally controlled indoor enviroment with really good lights, co2 and everything to simulate the Andes.

Poppies on the other hand are very hardly, look where 90% of therm are cultivated in the world; fucking Afghanistan. people all over the world can grow them, and I personally know of several western seed retailers that sell a lot of the seed to little old ladies. The only problem with papaver soniferum is if you grow a giant field and some Narco swine comes banging and says HEY LOOK AT ALL THESE ALKALOIDS WHAT ARE YOU UP TO, plus who the fuck grows fields of poppies in north america/uk?, I'm sure a helicopter could spot that quite easily... and its not like cannabis where you can put 10gallon grow bags sideways 20feet apart from eachother for 2miles.

The way of the future kids... is the Iran Poppy. The US government briefly considered growing warehouses full of the stuff and funding massive fields... but wait.. there is no opium or opiate alkaloids in this poppy.
Just Thebaine
what the fuck is thebaine?
Well what the fuck is Codiene, Oxycodone and the Thebacon
yeah Thebacon. there is an Opiate called Thebacon, look it up.

Thebaine from Iran Poppies, grow a field, narco swine asks what your doing say mail order flower business and whats a poppy. Even a small 40x50 plot will yield enough material to make an ounce of oxycodone at the end of the week.
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Edward Mangerwill - Thu, 22 May 2014 09:00:58 EST ID:Mj7GCD93 No.74749 Ignore Report Quick Reply
chew it with a pinch of baking soda, then you'll get a buzz going.
Sophie Bardstone - Wed, 04 Jun 2014 20:29:59 EST ID:VGm487Y7 No.74785 Ignore Report Quick Reply
it is. the only tropical climate in the US is hawaii, and that wouldnt be any better than south america

I think I finally figured it out. by Hamilton Pickwell - Mon, 02 Jun 2014 07:07:35 EST ID:WnzxeTmO No.74770 Ignore Report Reply Quick Reply
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Now in the beginning in the singularity before the big bang all of everything that is anything was in one singular point, right? That’s all the rage with the science people nowadays. Einstein demonstrated that space and time are basically the same thing, and we know that matter and energy can be interconverted, so check this. Fast forward to the end of the universe. When the universe experiences heat death due to eternal expansion and stars dying out, all matter has 0 energy and ceases to move. When it all ceases to move, time stops. When time stops, space becomes undefined because they are inextricably linked. When space becomes undefined, matter becomes undefined because space and matter are inextricably linked. When matter becomes undefined, matter converts to energy because there is no space to define its proportions as a physical substance. When all matter becomes energy, all borders between anything are gone. This means everything is now in one singular 'place'. It doesn't matter how big or little this place is because there are no borders or definitions it is only free energy. Then, the big bang is not only an expansion of space and time, but a contraction of free unrestrained energy into matter. The universe will actually become a singularity again by expanding and diluting itself until it reaches a zero point energy state and heat death, where matter will cease to exist and decay into energy.

Dark matter? Dark energy? Well, try this on for size…

Dark matter holds the universe back, dark energy pushes the universe out. It's simple. The balance of dark matter and dark energy determines the temporal duration of the universe until heat death and hard-reboot. It's a predetermined measurement of how long the universe will last.

And then matter/energy just determines the amount of stuff. (Energy or physical or… miscellaneous.)

And Space/Time determines the amount of space there is (accounting for changing amounts of space over time).
Shit Chublingpadge - Mon, 02 Jun 2014 12:34:23 EST ID:ZaxrFnTc No.74772 Ignore Report Quick Reply
And do you have any theoretical physics or empirical observations to back any of this up?
Edward Sessleham - Mon, 02 Jun 2014 18:54:22 EST ID:Fq5opoER No.74775 Ignore Report Quick Reply
Testable hypothesis or gtfo.
Emma Fendlepedging - Mon, 02 Jun 2014 19:05:05 EST ID:cM6rhCgJ No.74776 Ignore Report Quick Reply
I think he has a nifty idea. Now he just needs to spend the rest of his life mathing it out
Fuck Blytheforth - Tue, 03 Jun 2014 10:31:52 EST ID:TODCHygx No.74778 Ignore Report Quick Reply
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The only interesting idea presented here you stole from Roger Penrose. The rest is just garbage.

Low yield nukes by Angus Neshwell - Fri, 23 May 2014 06:43:15 EST ID:sBVjj75A No.74752 Ignore Report Reply Quick Reply
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>The smallest nuclear weapons actually deployed have had yields around 10 tons (like the W54), and have been intended for short range tactical or nuclear demolition use (e.g. blowing up roads and bridges).

>The absolute minimum possible mass for a bomb is determined by the smallest critical mass that will produce a significant yield. Since the critical mass for alpha-phase plutonium is 10.5 kg, and an additional 20-25% of mass is needed to make a significant explosion, this implies 13 kg or so. A thin beryllium reflector will reduce this, but the necessary high explosive and packaging will add mass, so the true absolute minimum probably lies in the range of 10-15 kg.

>The W54 warhead used in the Davy Crockett had a minimum mass of about 23 kg, and had yields ranging from 10 tons up to 1 kt in various mods (probably achieved by varying the fissile content). The warhead was basically egg-shaped with the minor axis of 27.3 cm and a major axis of 40 cm. The W-54 probably represents a near minimum diameter for a spherical implosion device (the U.S. has conducted tests of a 25.4 cm implosion system however).

Low yield mini nukes. And if you're fretting OMG what if these fell in the wrong hands -

they already have. Pic related.
2 posts omitted. Click Reply to view.
Jenny Billingfuck - Wed, 28 May 2014 13:29:35 EST ID:4kkjr9wQ No.74757 Ignore Report Quick Reply
Well this thread is /tinfoil/ bullshit, so yeah.
Walter Hoffingteg - Sun, 01 Jun 2014 12:18:28 EST ID:Nbu7dGdg No.74765 Ignore Report Quick Reply
THis isn't a /chem/ topic though. Take it to /pol/ or /n/.
Edwin Cribberwater - Mon, 02 Jun 2014 02:14:48 EST ID:LJNmkfOb No.74769 Ignore Report Quick Reply

Fucking nukes is Physics so it belongs in /chem/ which is a general science board.

Here's some papers on Dimona and detecting nukes:
Henry Chankincocke - Mon, 02 Jun 2014 10:48:14 EST ID:Nbu7dGdg No.74771 Ignore Report Quick Reply
Can you at least try to discuss something rather than just posting links? Are you asking something? Do you have a point to make? I'm not gonna read some random-ass papers because you said to.

Are you just saying that tactical nukes have been used? If so, could you find something better written? The articles you've posted are very rambling. And is the second link saying that 9/11 was caused by nukes? That's asinine. I'm sympathetic towards /tinfoil/ers, but it seems pretty obvious that 9/11 didn't use nukes. Like you said,
>people have been filming nuclear explosions for a long time, that's how we know what they look like.
And 9/11 didn't look like a nuke.

Edwin Drodgebury - Mon, 02 Jun 2014 18:29:48 EST ID:e4BjoeoE No.74774 Ignore Report Quick Reply
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It's for discussing nukes in general and where they are field tested.

Davy Crocket nuke is pretty small.

They used that kind of nuke on Syria, fired by artillery.

> The Mk-54 weighed about 51 lb (23 kg), with a yield equivalent to somewhere between 10 or 20 tons of TNT

Chemistry Literature, I have access by Sophie Cluzzlestock - Sat, 02 Nov 2013 08:25:25 EST ID:S6pQ/eNz No.73238 Ignore Report Reply Quick Reply
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Hi, my university offers access to databases of most chemical journals and archives for free. I can upload pdfs that would otherwise cost $ to purchase. Any requests?

I'll upload them here.
58 posts and 3 images omitted. Click Reply to view.
Cyril Tootspear - Wed, 23 Apr 2014 20:47:23 EST ID:0xjykuyo No.74581 Ignore Report Quick Reply
Cyril Tootspear - Wed, 23 Apr 2014 21:39:12 EST ID:0xjykuyo No.74582 Ignore Report Quick Reply
Are you thinking of building one? Go for it.

> The magnet required for this large a sample needed to have at least a 5 (125 mm) free bore in order to hold an NMR probe large enough to accommodate this sample...
>The magnet used in the spectrometer described here has a 5.5 (140 mm) free bore and weighs 300 lb (136 kg). We also have a standard H magnet that was part

Holy shit, that's a huge NMR for just 20MHz. I've used a permanent magnet 27MHz tabletop NMR (of course, with smaller samples).

>The whole system less the magnet can be built at a cost of below $20 000.
I'm pretty sure the kit for the low-freq. NMR I used was about $10,000 two years ago. Although it just used an oscilloscope for the output, so maybe a lot of the cost is in the data processing shit.

That's a neat article and has lots of good ideas (like the antifreeze pumped around the magnets), but it was written for academics who are around lots of NMRs already, and can rip off a 300lb magnet from a broken NMR. It didn't even mention what the gradient coils are (are they part of the 300 pound thing?).

Check this shit out for a more doable DIY NMR: . That's the dinky one I used. The company also sells an NMR that works using the earth's magnetic field. That one is probably good for comparing T1 times between oil and water, but that's it. Of course, that sounds like a great way to start.
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Cyril Tootspear - Wed, 23 Apr 2014 21:43:34 EST ID:0xjykuyo No.74583 Ignore Report Quick Reply
Walter Hoffingteg - Sun, 01 Jun 2014 12:23:31 EST ID:Nbu7dGdg No.74767 Ignore Report Quick Reply
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I've been using this with GREAT success:
Walter Hoffingteg - Sun, 01 Jun 2014 12:23:56 EST ID:Nbu7dGdg No.74768 Ignore Report Quick Reply

TIME by Edwin Peppersuck - Sat, 19 Apr 2014 07:41:30 EST ID:vxCf2gKS No.74545 Ignore Report Reply Quick Reply
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My knowledge on physics is quiet scarce, so the details might not be correct, please try to focus on the general idea.

When you observe something, you're picking up the light that bounces of an object.
Light travels at the speed of light.
The closer something comes to the speed of light the slower it moves and the more dense it becomes.
At the speed of light, time stops.
When you're at two opposing ends with equal distances of an object, you might see a different perspective, but you see the same object, in the same point of time.
This information travels to you with the speed of light, and this time stops.
Therefor time is not constant and you actually see two different copies of the same thing.. or something...

Can anybody explain to me how this works and if I'm completely wrong?
Hedda Nazzlebanks - Sat, 19 Apr 2014 14:30:42 EST ID:E2i1a/eH No.74546 Ignore Report Quick Reply
My understanding of relativity isn't perfect, so I'll probably make some mistakes.
>The closer something comes to the speed of light the slower it moves and the more dense it becomes.
Well, the closer something goes to the speed of light, it's still getting faster. For the thing traveling, space gets shorter and shorter along the axis its moving.
> but you see the same object, in the same point of time... Therefor time is not constant and you actually see two different copies of the same thing.. or something...

I'm not sure what you're trying to say here, but it's useless/confusing to talk about "the same time" like that. Instead, think about "Simultaneous events". You're right to try wrapping your head around it by bouncing light between two points. Simulataneous events are defined by bouncing light between two clocks. If both clocks measure the same amount of time for the bounce, then the clocks are "synchronous." That is, an event that happens at 1 second on clock A simultaneously happens at 1 second on clock B. Clocks are synchronous when they're both still, or moving at the same speed in the same direction (which is relatively the same thing, if you think about it). When one clock has a different speed/direction than the other, then the clocks won't be synchronous. For example, if one clock is moving at the speed of light, then a stationary clock might have every *other* second be a synchronous event with every second of the moving clock.

It's important to note that this also depends on what you make as your "reference frame". In the example I just gave, you could think of it as DEFINING the stationary clock as stationary according to the reference frame.

Hopefully someone who knows what they're talking about will set us straight.
Hedda Nazzlebanks - Sat, 19 Apr 2014 14:41:51 EST ID:E2i1a/eH No.74548 Ignore Report Quick Reply
> For example, if one clock is moving at the speed of light,
I meant to say "near the speed of light". Which brings me to what you said:
>At the speed of light, time stops.
This isn't really true. There might be theories for what happens when something with mass goes the speed of light, but physicists usually just say it's impossible. If it is possible, then there's no generally accepted theory for what happens. Photons and some other particles go the speed of light because they have ZERO mass. An electron has mass, so it cannot go the speed of light.
Jenny Blandlefack - Wed, 23 Apr 2014 04:04:45 EST ID:iyn6pQgs No.74572 Ignore Report Quick Reply
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Try the game "A Slower Speed of Light", it allows you to experience special relativity.
Walter Hoffingteg - Sun, 01 Jun 2014 12:22:40 EST ID:Nbu7dGdg No.74766 Ignore Report Quick Reply

Mirrors by Phyllis Gunderwat - Sun, 23 Mar 2014 22:03:20 EST ID:CT0cbrIE No.74395 Ignore Report Reply Quick Reply
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How can mirrors be real, if our eyes aren't real?
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Jarvis Cocklehood - Sun, 04 May 2014 05:28:47 EST ID:rah7DDRz No.74654 Ignore Report Quick Reply
Edward Mangerwill - Thu, 22 May 2014 09:08:26 EST ID:Mj7GCD93 No.74750 Ignore Report Quick Reply
we need O2 and other good stuff in air, water is H2O no bueno
Ebenezer Brizzlemit - Wed, 28 May 2014 18:56:59 EST ID:cM6rhCgJ No.74760 Ignore Report Quick Reply

thanks edward.
Hugh Cunderhudging - Fri, 30 May 2014 05:25:49 EST ID:Mj7GCD93 No.74761 Ignore Report Quick Reply
but he wanted an explanation of marxist pinheads!?!?!?!?£
Charlotte Clizzlestone - Sat, 31 May 2014 20:37:08 EST ID:UR/i6ew3 No.74762 Ignore Report Quick Reply

Dog-Vision by G - Mon, 12 May 2014 17:32:46 EST ID:YDQsbS20 No.74695 Ignore Report Reply Quick Reply
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Can someone give me a relatively quick explanation as to how people came to the conclusion that dogs see in and black+white+ some shades of blue? Not debating the scientific fact just curious as to what methods we used to come to that conclusion..

I've been aware Dogs see in black+white since I was about 4 or 5 from watching that episode of The Simpsons where it shows life from their dog's perspective, but never knew how the hell people were able to figure this out exactly. I tend to spend/waste a fair amount of time pondering what the world looks like through the eyes of animals/aliens, impossible colors by human standards and such.
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Faggy Trotfuck - Sat, 17 May 2014 23:31:58 EST ID:UcjD537E No.74732 Ignore Report Quick Reply
The point is a dog is capable of forming that bond with another individual. Yes it's a "learned trait", but that's inextricable from the idea of a relationship. A relationship is learned. Dogs are capable of learning relationships.
Edward Mangerwill - Thu, 22 May 2014 08:52:50 EST ID:Mj7GCD93 No.74748 Ignore Report Quick Reply
dogs have similar photoreceptors to us rods and 2 kinds of cones, but their eyes are primarily rods which are better for night vision but less color acuity. we have many more photoreceptors in our eyes, with primarily cones in the fovea, so good color vision. Dogs have the same smell receptors as we do, just a lot more of them, kinda puts things in perspective.
Phoebe Nablingstun - Sun, 25 May 2014 14:25:30 EST ID:oRLfV4+h No.74755 Ignore Report Quick Reply
Some dogs are highly intelligent. My german sheppard, for instance, listens to me intently when I talk and tries to pick out words she can understand. To a degree, she can understand human language. Certainly not dumb, to some extent.

Some dogs are also dumb as fuck. Its hard to generalize an entire fucking species people. I mean, just look at humans. Some humans are capable of intense abstract and mathematical thought, while others can't even spell the words of a simple twitter post correctly. Our intelligences vary so much. Same with dogs.

Also elephants kick ass.
Phoebe Bankinwure - Wed, 28 May 2014 13:50:58 EST ID:iHZcrM0s No.74758 Ignore Report Quick Reply
> but their eyes are primarily rods which are better for night vision

While this is correct, as with cats, they higher density of rod cells are for higher contrast in vision, for sensing movement.

As active hunters, they are less concerned with color as they are with motion of prey. Us munkeys have a well developed perception of color due to our old foraging days. 'Don't eat that bright yellow bug, shit will kill you.' as opposed to, 'bark, im a wild dog, did you see that, i though i just sa...SQUIRRELSQUIRRELSQUIRRELGETITGETITGETIT'
Simon Nobblefield - Wed, 28 May 2014 15:16:56 EST ID:Nbu7dGdg No.74759 Ignore Report Quick Reply
Also, they have a reflective layer of cells behind the retina, so light passes through the retina twice. That's why lots of animals have those shiny eyes. It helps quite a bit with night vision.

Process for dextrose dilution by Faggy Mommerfield - Tue, 20 May 2014 09:22:48 EST ID:EsrXPHEw No.74741 Ignore Report Reply Quick Reply
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Hello chem,

I have a specific chemical that I'd like to ingest via intranasal roa. It is active in the 1mg range, which isn't very fun to blow.

I want to dilute it 1:100 with dextrose or a similar nontoxic filler, so that each dose takes up about 100mg. More fun to blow that way and much much safer since my mg scale is wonderful... But I get much less accurate measures under 2-3mg.

What's the best way to homogenize two powders of different densities together? Hotspots won't kill me (this isn't fentanyl or anything) but would be frustrating, wasteful and potentially dangerous to others using this product. I will know my starting weight of product as 100mg.

The chemical is etilozam, and is not soluble in water.

Edward Mangerwill - Thu, 22 May 2014 08:45:32 EST ID:Mj7GCD93 No.74747 Ignore Report Quick Reply
flour sifter is used for this kinda thing, anyone who bakes will have one, but not really useful for tiny amounts.....
maybe put it in a jar and shake it
or just take tiny knifetip bumps.
Syllogism - Fri, 23 May 2014 04:27:53 EST ID:stqlrmZK No.74751 Ignore Report Quick Reply
Decrease solvent polarity brah.

Water Bottle Fizzles by Doris Bleddlestone - Tue, 20 May 2014 10:48:23 EST ID:dtyqJQcU No.74743 Ignore Report Reply Quick Reply
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Does anyone know why your water bottle makes fizzling noises after you close the lid to a certain tightness?

Is there some type of gas in there?
Faggy Mommerfield - Tue, 20 May 2014 12:57:49 EST ID:EsrXPHEw No.74744 Ignore Report Quick Reply
As the water warms and the contents of the bottle increase in temperature, pressure is created.

Tall Fescuca by Clara Geshbin - Sun, 18 May 2014 19:28:07 EST ID:nB4YYFMr No.74734 Ignore Report Reply Quick Reply
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Does anyone know anything about Festuca arundinacea (Tall Fescue) involving the cultivation of ergot alkaloids? Couldn't this by a more viable source for ergot alkaloids vs the traditional wheat method?
Cornelius Cabblewell - Mon, 19 May 2014 07:35:27 EST ID:T5wS+R1s No.74737 Ignore Report Quick Reply
Yes its true all you need is Tall Fescue seed with a high level (>90%) endophyte infection. You either have to find/purchase seed with high levels already, or you have to isolate a wild strain of tall fescue and "cultivate" the endophytes and increse the level of infection. there is a lot of literature on the subject, there was a university student that was able to increse endophyte levels by growing patches of the grass in a greenhouse

primarily the alkaloids you will be producing in this grass are Lolines and Ergovaline, there much more Loline than there is Ergovaline so some sort of fractional separation is neccesary in the isolation process

The ergot alkaloids are found roughly 0.3mg/kg in the leaf blades and 2.8mg/kg in the leaf sheaths according to one paper, another says they can be found 0.1ug-15ug per gram of material. Obviously these are very low numbers.

to make a pound of ergovaline salt one would need a LOT of grass, solvent and time. I'm not sure how well this would work for small scale. I'm thinking a 40x50 plot minimum of grass.

Heres some papers on the subject

"Variability of Ergovaline in Seeds and Straw and Endophyte Infected Genotypes of Tall Fescue"
"Occurence of Peptide and Clavine Ergot Alkaloids in Tall Fescue Grass"

Is it better than growing a bunch of claviceps? who knows. I have read you can get 20pounds of claviceps from an entire field of male rye (the one that has the open stomata the longest)
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