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Contamination by Graham Gimmlehood - Tue, 16 Jan 2018 14:01:09 EST ID:aWb7Y/oB No.145721 Ignore Report Quick Reply
File: 1516129269999.jpg -(53371B / 52.12KB, 362x512) Thumbnail displayed, click image for full size. 53371
I'm having issues with contamination, specifically green mold. I'm using grain bags, washing hands before inoculation, used the alcohol swabs, flame sterilized the needle etc, but it just keeps happening, are there any more ways to prevent this?
press !//CEObOMBY - Tue, 16 Jan 2018 18:03:26 EST ID:EEDCh3z1 No.145723 Ignore Report Quick Reply
making the spore syringes and the bags yourself or are they bought?
how do you seal the bags after innoculation?
Graham Gimmlehood - Tue, 16 Jan 2018 18:05:21 EST ID:aWb7Y/oB No.145724 Ignore Report Quick Reply

I'm buying them online pre sterilized, the injector ports are the self healing kind
press - Tue, 16 Jan 2018 19:37:02 EST ID:9WfOuCGW No.145727 Ignore Report Quick Reply
same for the syringes i assume.
and the mold shows up in the bag or later on?
Martha Brommleridge - Wed, 17 Jan 2018 04:32:33 EST ID:aWb7Y/oB No.145731 Ignore Report Quick Reply

Mold shows up about a day or 2 after the white mycelium starts to spread
Martha Brommleridge - Wed, 17 Jan 2018 05:33:51 EST ID:aWb7Y/oB No.145732 Ignore Report Quick Reply

Spores were bought online from 2 separate places. Really failing to see how contamination is happening. The first one was typical green looking mold, but my current bag has almost leaf green shit growing in it.
press !//CEObOMBY - Wed, 17 Jan 2018 15:56:57 EST ID:Ejt2rxtj No.145734 Ignore Report Quick Reply
i highly doubt a problem with the grain bag itself.

no experience with bought spore syringes but i also highly doubt a vendor would fuck that up, but it sounds like it.

could you walk me through your procedure a bit more thoroughly?
Martha Brommleridge - Wed, 17 Jan 2018 16:49:32 EST ID:aWb7Y/oB No.145735 Ignore Report Quick Reply

I wash my hands, place the bag on a tray wiped down with iso, flame the needle and wipe with an alcohol swab, swab the injector port and inject. Don't push the needle very far into the bag.

I'm going to construct a clean air enclosure for my next attempt, and bought the bag from a different vendor. Also using BRF instead of rye.
Esther Bunhood - Fri, 26 Jan 2018 11:28:57 EST ID:aWb7Y/oB No.145751 Ignore Report Quick Reply

So after inoculating my 3rd bag in a still air environment, wearing gloves and a facemask, having scrubbed the surfaces I'm working on with iso, flame sterilizing my needle and using a self healing injector port, I've noticed cobweb growing in the 3rd bag.

All 3 attempts have been clean, but contamination still occurs. Very confused as to whats going on, and getting pretty pissed off with it. Any idea whats happening?
press !//CEObOMBY - Fri, 02 Feb 2018 15:50:48 EST ID:U9XwZQD5 No.145757 Ignore Report Quick Reply
obviously you cant state which vendor you bought off but at this point i would consider telling them about your experience. provided youve been truthful in this thread it really does sound like a problem with the syringe altough id expect one particular contam. but then again with mycology one fuck up rarely comes alone.

i cant really give you anything at this point. if you had agar and an sab you could test the syringes yourself
Betsy Grimforth - Thu, 08 Mar 2018 06:39:30 EST ID:f+64uMjk No.145817 Ignore Report Quick Reply
That image is dope
Charles Pamblemud - Sun, 18 Mar 2018 23:55:42 EST ID:Uzi0n9n1 No.145846 Ignore Report Quick Reply
1521431742899.jpg -(169110B / 165.15KB, 1024x720) Thumbnail displayed, click image for full size.
Read about non-sterile mushroom growing technique, given the right environment, the mushroom will have no problem out competing other micro organisms
Eugene Gimblenene - Mon, 02 Apr 2018 13:05:49 EST ID:vjKZCFX2 No.145871 Ignore Report Quick Reply
Sterilize your substrate on your own, it's easy and you can exclude one fuck-up factor out of your equation. That leaves you with the spores, try different vendors and maybe try to get both, syringes and spore prints. You just need one successful run to keep this going for as long as you want to
Simon Soddleman - Tue, 03 Apr 2018 05:12:54 EST ID:aWb7Y/oB No.145872 Ignore Report Quick Reply

I tried two seperate vendors for both spores and substrate. Althought I got the spores off ebay so thats probably why they weren't up to standard. Its very hard to find decent vendors here even online.

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