|>> || 1610746573584.jpg -(138252B / 135.01KB, 1080x608) Thumbnail displayed, click image for full size. >>147504 |
so thats a spore syringe from a reputable vendor or are you going to make your own from a spore print?
ive never ordered a syringe since shipping those would make me nervous, but from what ive read the syringes of reputable vendors e.g. shroomery sponsors are pretty clean.
assuming that youre going for PF tek, id suggest the following:
- dont get some stupid air stone bubbler set up. a shotgun fruiting chamber with perlite works well. other fruiting chambers can work too for PF / BRF cakes, but ive never tried them.
- a pressure cooker isnt necessary just yet, but i recommend it. if you plan to go beyond PF tek and just the occasional grow, try to gauge if youd like to invest the money for a bigger PC. should you be in north america a presto 16 quart or 23 quart pressure cooker might be easy to come by cheap on ebay, euro cukcs such as myself arent as lucky.
- if you have an electric grinder, make your own brown rice flour instead of buying it. a bit coarser is better than really fine flour.
- id use coarser vermiculite, or if you feel like complicating things a bit use fine verm for the actual cake and coarser verm for the protective dry layer on top. the finer verm absorbs water a bit better in my experience but isnt quite as good for the protective layer, since it can shift around more easily
- absolutely use a still air box
- do a "dry run" i.e. load up your still air box with empty jars and practise the movements, get a feel for aseptic technique. try to move smoothly and visualize steps beforehand.
- its not strictly necessary to shower, brush your teeth, wear fresh clothes, a hair net but i like to employ that practise in order to have a ritual that gets me into a good and controled mind set. id really recommend a surgical face mask though.
- unless youre going to store the colonizing jars in a room below 16 °C you dont have to incubate the jars. ideally youd have them in a place where they get a bit of ambient light and a bit of fresh air, just on a shelf in next to your desk works fine. you could place them on a higher shelf inside a cardboard box from which youve removed the top if you want to keep it a bit stealthier. in my experience incubation has introduced for problems than it helped.
- dont move them too much and if you inspect them ( because lets face it, everybody will anxiously look for any sign of growth ) try not to tilt them, so that the protective verm layer doesnt get tumbled around.
- give them enough time to form small pins while still inside the jar before "birthing" them
- rinse the cakes under cold water when you birth them and roll them in verm. let them sit in the shotgun fruiting chamvber for a few hours before misting them, that seems to help the verm coating stick to them
- after the first flush, let the cakes sit in the shotgun fruiting chamber for a day or two before dunking them. dont do a second roll in verm after youve dunked them
- try to observe what ideal surface conditions look like
- dont get a hygrometre, theyre not necessary
more broadly speaking:
- dont be afraid to start agar work.
- glass petris are shit. they crack, theyre slippery, you have to sterilize them anyways. gamma radiated polystyrene dishes are the way to go. ive always used ones without ventilation groves, but some people swear by them.
- dont try liquid cultures untill youre pretty comfortable in your agar work.
- dont be afraid to start bulk grain grows once youve done a few runs with PF or feel confident enough in your technique and judgement of surface condition. this comes with a few caveats but its a lot more time, space, and eventually cost efficient than PF.
- dont listen to assholes on youtube. most are absolute shit ime.
and i wish i had known about unmodded monotubs and shoe boxes when i started doing bulk grains instead of drilling stupid holes into perfectly fine and inscopicous plastic totes ( not to insult monotubs, theyre great)
please excuse any typos or grammatical errors. im also a bit rusty, but am kinda proud of myself that as of recently only 1 out of 52 grain jars contaminated. suprisingly enough it wasnt even trichoderma but probably a black pin mold.