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Harm Reduction Notes for the COVID-19 Pandemic

wheresmymotherfucking biology board

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- Thu, 31 Dec 2015 22:24:52 EST IaY1gFOQ No.77484
File: 1451618692877.jpg -(178474B / 174.29KB, 640x480) Thumbnail displayed, click image for full size. wheresmymotherfucking biology board
hey, fuckin, if i run a current through some shit, like a strong fuckin "im gonna connect extremely powerful device A to mains outlet but the wire's going through thistank of water

will it kill everything? even the microbes? even the tiny superhero antman?
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Sophie Wegglenidge - Sat, 02 Jan 2016 02:20:49 EST LB+wr31L No.77489 Reply
This thread is bad and you should feel bad.
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James Chommerbury - Tue, 05 Jan 2016 00:51:38 EST GEEvdbi2 No.77498 Reply
Hopefully it only kills you
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Ebenezer Sonkinman - Wed, 03 Feb 2016 13:24:04 EST yvLCKMRO No.77614 Reply
The OP is stupid, the question is not. Does anyone actually know whether microbial life can survive high voltage?
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Sophie Chonkinchetch - Thu, 04 Feb 2016 11:53:58 EST wE3/tzia No.77617 Reply
>>77614
  1. Inoculate appropriate (liquid) culture medium with some non-pathogenic, non-sporulating bug.
  2. Split the broth between two sterile electrolytic cells (to rule out the influence of the electrodes or whatever).
  3. Apply suitably high voltage to one of the cell, for a suitable amount of time.
  4. Wait for the bugs to multiply, then compare optical density.
  5. Repeat.
  6. Report.
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Hugh Battinggold - Mon, 08 Feb 2016 01:10:15 EST FHFwCltH No.77635 Reply
Presumably it would eventually kill microbes.
One common means of transforming (aka introducing plasmids) into bacteria is elctroporation, which is essentially zapping bacteria with pulses of electricity. These pulses generate small pores in their membranes, into which plasmid DNA slips in.

However this is done in a highly controlled setting, high intensity bursts, and even that produces holes in their membranes, and even that kills alot of the bacteria. Its assumed that even if only 1% survives, in a culture of >1million cells thats no big deal, you still have 10000 cells that will produce viable colonies.

Now, after that little tangent, electric currents are used as a form of chromotography known as electrophoresis. Essentially when you run DNA or protein gels you separate by size based on charge. Since most molecules in the cell are either negative or positively charged, you would essentially tear the cell apart based on the charge of molecules within it. Even the membrane would be torn apart as the negatively charged head groups are pulled toward the cathode, with basic proteins pulled toward the anode.

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